A simple precise and accurate stability indicating RP-HPLC method has been developed for estimation of metolazone in pharmaceutical dosage form. A stability indicating assay accurately measures the active ingredients, without interference from degradation product. Stability study and degradation kinetics are an integral part of the quality control of the drug or medicinal product on an industrial level. Forced degradation of drug substance and drug product according to the ICH guideline was studied. In RP-HPLC method methanol:water (80:20% v/v) was used as a mobile phase. The Hiber 100-5 C18 column, 250 mm L × 4.6 mm in size was used as a stationary phase for separation of drug with other degraded product. The flow rate was 0.8 ml/ min, detection wavelength was 236 nm. The linearity was observed in range of 3 µg/ml -7 µg/ml. The peak purity was found to be more than 0.990 the assay of the drug was found in acceptance range. The degradation product peak was well resolved from the pure drug peak with significant difference in their retention time, so that developed method could be used for routine analysis and stability studies.
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